Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry
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Authors
Krueger, Brett
Shetty, Anand
Esqueda, David
Bentley, Leah
Hooper, Jonathan
Zumbuhl, Amelia
Butkus, Michael
Pfluger, Andrew
Issue Date
2024-04-26
Type
Journal articles
Language
Keywords
Respirometry , Biokinetics , Anaerobic Co Digestion , Food Scrap Waste , Waste Activated Sludge , Methan Production , Substrate Utilization , Biokinetic Parameters
Alternative Title
Abstract
The use of respirometry to study the biokinetics of microbiota treating wastewater or digesting wastewater sludges has become more prevalent over the last few decades. The use of respirometry to examine the biokinetics of anaerobic microbiota co-digesting organic waste streams such as wastewater sludge and food scrap is an area of active research. To date, no visualized protocol has been published on the topic. Accordingly, in this protocol, we configured a respirometer to measure methane production and flow rate over time using three different food-to-microorganism (F:M) ratios and food scrap waste and waste-activated sludge as substrates. The resulting data, coupled with substrate utilization measurements, provides the basis for understanding how different substrate concentrations influence the rate at which anaerobic microbiota produce methane. Additionally, this protocol presents a method to develop biokinetic parameters (e.g., methane production rate constant and yield). Others can use this respirometry protocol to examine organic degradation under anaerobic conditions and develop microbial parameters.
Description
Citation
Krueger, B., Shetty, A., Esqueda, D., Bentley, L., Hooper, J., Zumbuhl, A., Butkus, M., Pfluger, A. Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry. J. Vis. Exp. (206), e66485, doi:10.3791/66485 (2024).
Publisher
JoVE
License
Journal
Volume
Issue
PubMed ID
DOI
ISSN
1940-087X