Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry

dc.contributor.authorKrueger, Brett
dc.contributor.authorShetty, Anand
dc.contributor.authorEsqueda, David
dc.contributor.authorBentley, Leah
dc.contributor.authorHooper, Jonathan
dc.contributor.authorZumbuhl, Amelia
dc.contributor.authorButkus, Michael
dc.contributor.authorPfluger, Andrew
dc.date.accessioned2024-10-29T16:28:49Z
dc.date.available2024-10-29T16:28:49Z
dc.date.issued2024-04-26
dc.description.abstractThe use of respirometry to study the biokinetics of microbiota treating wastewater or digesting wastewater sludges has become more prevalent over the last few decades. The use of respirometry to examine the biokinetics of anaerobic microbiota co-digesting organic waste streams such as wastewater sludge and food scrap is an area of active research. To date, no visualized protocol has been published on the topic. Accordingly, in this protocol, we configured a respirometer to measure methane production and flow rate over time using three different food-to-microorganism (F:M) ratios and food scrap waste and waste-activated sludge as substrates. The resulting data, coupled with substrate utilization measurements, provides the basis for understanding how different substrate concentrations influence the rate at which anaerobic microbiota produce methane. Additionally, this protocol presents a method to develop biokinetic parameters (e.g., methane production rate constant and yield). Others can use this respirometry protocol to examine organic degradation under anaerobic conditions and develop microbial parameters.
dc.description.sponsorshipDepartment of Geography and Environmental Engineering
dc.identifier.citationKrueger, B., Shetty, A., Esqueda, D., Bentley, L., Hooper, J., Zumbuhl, A., Butkus, M., Pfluger, A. Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry. J. Vis. Exp. (206), e66485, doi:10.3791/66485 (2024).
dc.identifier.doi10.3791/66485
dc.identifier.issn1940-087X
dc.identifier.urihttps://app.jove.com/t/66485/measuring-biomethane-potential-food-scrap-waste-anaerobically
dc.identifier.urihttps://hdl.handle.net/20.500.14216/1625
dc.publisherJoVE
dc.relation.ispartofJournal of Visualized Experiments
dc.subjectRespirometry
dc.subjectBiokinetics
dc.subjectAnaerobic Co Digestion
dc.subjectFood Scrap Waste
dc.subjectWaste Activated Sludge
dc.subjectMethan Production
dc.subjectSubstrate Utilization
dc.subjectBiokinetic Parameters
dc.titleMeasuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry
dc.typeJournal articles
local.USMAemailbrett.krueger@westpoint.edu
local.peerReviewedYes
oaire.citation.issue206

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