Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry
dc.contributor.author | Krueger, Brett | |
dc.contributor.author | Shetty, Anand | |
dc.contributor.author | Esqueda, David | |
dc.contributor.author | Bentley, Leah | |
dc.contributor.author | Hooper, Jonathan | |
dc.contributor.author | Zumbuhl, Amelia | |
dc.contributor.author | Butkus, Michael | |
dc.contributor.author | Pfluger, Andrew | |
dc.date.accessioned | 2024-10-29T16:28:49Z | |
dc.date.available | 2024-10-29T16:28:49Z | |
dc.date.issued | 2024-04-26 | |
dc.description.abstract | The use of respirometry to study the biokinetics of microbiota treating wastewater or digesting wastewater sludges has become more prevalent over the last few decades. The use of respirometry to examine the biokinetics of anaerobic microbiota co-digesting organic waste streams such as wastewater sludge and food scrap is an area of active research. To date, no visualized protocol has been published on the topic. Accordingly, in this protocol, we configured a respirometer to measure methane production and flow rate over time using three different food-to-microorganism (F:M) ratios and food scrap waste and waste-activated sludge as substrates. The resulting data, coupled with substrate utilization measurements, provides the basis for understanding how different substrate concentrations influence the rate at which anaerobic microbiota produce methane. Additionally, this protocol presents a method to develop biokinetic parameters (e.g., methane production rate constant and yield). Others can use this respirometry protocol to examine organic degradation under anaerobic conditions and develop microbial parameters. | |
dc.description.sponsorship | Department of Geography and Environmental Engineering | |
dc.identifier.citation | Krueger, B., Shetty, A., Esqueda, D., Bentley, L., Hooper, J., Zumbuhl, A., Butkus, M., Pfluger, A. Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry. J. Vis. Exp. (206), e66485, doi:10.3791/66485 (2024). | |
dc.identifier.doi | 10.3791/66485 | |
dc.identifier.issn | 1940-087X | |
dc.identifier.uri | https://app.jove.com/t/66485/measuring-biomethane-potential-food-scrap-waste-anaerobically | |
dc.identifier.uri | https://hdl.handle.net/20.500.14216/1625 | |
dc.publisher | JoVE | |
dc.relation.ispartof | Journal of Visualized Experiments | |
dc.subject | Respirometry | |
dc.subject | Biokinetics | |
dc.subject | Anaerobic Co Digestion | |
dc.subject | Food Scrap Waste | |
dc.subject | Waste Activated Sludge | |
dc.subject | Methan Production | |
dc.subject | Substrate Utilization | |
dc.subject | Biokinetic Parameters | |
dc.title | Measuring Biomethane Potential of Food Scrap Waste Anaerobically Co-Digested with Waste-Activated Sludge Using Respirometry | |
dc.type | Journal articles | |
local.USMAemail | brett.krueger@westpoint.edu | |
local.peerReviewed | Yes | |
oaire.citation.issue | 206 |